Violaxanthin cycle kinetics analysed in vivo with resonance Raman spectroscopy

verfasst von

Matthias Koch, Serge Zagermann, Ann Kathrin Kniggendorf, Merve Meinhardt-Wollweber, Bernhard Roth

Abstract

Measuring the kinetics of the violaxanthin cycle imposes an experimental challenge. Traditionally, carotenoid analysis was carried out laboriously with high-performance liquid chromatography. In this work, we present the first in vivo approach to directly measure the kinetics of the violaxanthin cycle, using resonance Raman spectroscopy in combination with baseline correction and principal component analysis. Applying the new approach allows measuring thousands of data points as opposed to the few possible with chemical analysis over the course of a violaxanthin cycle kinetics experiment. In vivo analysis of the violaxanthin cycle is necessary to fully understand adaptation kinetics to varying light conditions, the knowledge of which is especially important for assessing the stress tolerance of plants in the wake of the increasing climate change. Three experiments on the green alga Dunaliella salina were performed, featuring both the light-to-dark and dark-to-light transitions response of the algae.

Organisationseinheit(en)

Hannoversches Zentrum für Optische Technologien (HOT)

Typ

Artikel

Journal

Journal of Raman spectroscopy

Band

48

Seiten

686-691

Anzahl der Seiten

6

ISSN

0377-0486

Publikationsdatum

05.2017

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Allgemeine Materialwissenschaften, Spektroskopie

Ziele für nachhaltige Entwicklung

SDG 13 – Klimaschutzmaßnahmen

Elektronische Version(en)

https://doi.org/10.15488/1214 (Zugang: Offen)
https://doi.org/10.1002/jrs.5102 (Zugang: Geschlossen)