Single-use membrane adsorbers for endotoxin removal and purification of endogenous polysialic acid from Escherichia coli K1
- authored by
- Ingo de Vries, Sarah Schreiber, Daniel Boßmann, Jens Kopatz, Harald Neumann, Sascha Beutel
- Abstract
Polysialic acid (polySia) is a promising molecule for various medical applications (e.g., treatment of inflammatory neurodegenerative diseases). In this study a complete production process for human-identical α-(2,8)-linked polySia was developed using a disposable bioreactor for cultivation of Escherichia coli K1 and single-use membrane adsorbers for downstream processing (DSP). The cultivation process was optimized to minimize complex media components and a maturation process after cultivation was established. The maturation led to further product release from the cell surface into the supernatant. Afterwards DSP was established using sodium hydroxide treatment combined with anion exchange membrane adsorbers for endotoxin and DNA depletion. After downstream processing the final product had neither detectable protein nor DNA contamination. Endotoxin content was below 3 EU mg
−1. Investigation of the maximal chain length showed no effect of the harsh sodium hydroxide treatment during DSP on the stability of the polySia. Maximal chain length was ∼98 degree of polymerization.
- Organisation(s)
-
Institute of Technical Chemistry
- External Organisation(s)
-
University of Bonn
- Type
- Article
- Journal
- Biotechnology Reports
- Volume
- 17
- Pages
- 110-116
- No. of pages
- 7
- Publication date
- 03.2018
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Biotechnology, Applied Microbiology and Biotechnology
- Sustainable Development Goals
- SDG 3 - Good Health and Well-being
- Electronic version(s)
-
https://doi.org/10.1016/j.btre.2018.02.001 (Access:
Open)
https://doi.org/10.15488/3197 (Access: Open)
