Correlative imaging and quantification of the tumor microenvironment of triple-negative-breast-cancer using lightsheet microscopy, scanning laser optical tomography, and TPEF

authored by

Hannes Kamin, Lena Nolte, Jochen Maurer, Elmar Stickeler, Dag Heinemann, Alexander Heisterkamp, Sonja Johannsmeier, T. Ripken

Abstract

The triple-negative breast cancer is an aggressive subtype that has a high rate of relapse and a poor five-year survival rate. The Tumor Microenviroment influences the behaviors such as proliferation, migration and the formation of metastasis. We present the complementary imaging and subsequent analysis of the Tumor Microenviroment using Lightsheet Microscopy, Scanning Laser Optical Tomography and TPEF. The samples were imaged in different size and resolution scales and the three-dimensional information is obtained using the SHG-signal of collagen and fluorescence of labeled key markers. The results presented here may help to create a patient specific model of breast cancer.

Organisation(s)

Abteilung Phytophotonik
Hannover Centre for Optical Technologies (HOT)
Institute of Quantum Optics

External Organisation(s)

RWTH Aachen University
Laser Zentrum Hannover e.V. (LZH)
NIFE - Lower Saxony Centre for Biomedical Engineering, Implant Research and Development

Type

Audiovisual publication

Publication date

06.03.2023

Publication status

Published

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.1117/12.2648373 (Access: Closed)