Cryopreservation of Dendritic Cells for Cancer Therapy

authored by

Tarek Deeb, Lena Zeh, Tim Sören Frederik Rittinghaus, Claudia Dettmar-Richardt, Simon Grammel, Birgit Glasmacher

Abstract

In recent decades, cell therapy has become increasingly important as a complementary approach to cancer treat-ment. Dendritic cell therapy shows great potential for the treatment of cancer. Better availability of this cell therapy should be ensured by cryopreservation. The project is dedicated to the investigation of a supply chain model in which equine dendritic cells derived from monocytes are first cultured, shipped and cryopreserved. Then, the samples are thawed and shipped back after three specifi c time periods corresponding to the real therapeutic process. In total, the quality, as well as the quantity (automatic cell counting as well as vitality determination) of ൱൰ samples from four diff erent animals, is evaluated before and after the cryopreservation process in terms of storage time, con-centration, and total volume. Here, a composition of ൯ mil-lion cells in ൫ ml seems to be the most suitable for the pro-cedure and provided better results (overall vitality ≥൱൲%). In addition, isotonic saline was found to have the potential to replace autologous plasma in cryoprotectants.

Organisation(s)

Institute of Multiphase Processes

External Organisation(s)

PetBioCell GmbH

Type

Article

Journal

Problems of cryobiology :scientific and theoretical journal

Volume

32

ISSN

0233-7673

Publication date

30.12.2022

Publication status

Published

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.15407/cryo32.04.300a (Access: Open)