Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp. SANK 73390
- authored by
- Annabel C. Murphy, Shu Shan Gao, Li Chen Han, Simon Carobene, Daisuke Fukuda, Zhongshu Song, Joanne Hothersall, Russell J. Cox, John Crosby, Matthew P. Crump, Christopher M. Thomas, Christine L. Willis, Thomas J. Simpson
- Abstract
The biosynthesis of the mixed PKS-FAS-NRPS hybrid antibiotic thiomarinol A was investigated using feeding studies to both wild type and mutant strains of the marine bacterium Pseudoalteromonas. Particularly interesting features of the pathway include assembly of the 8-hydroxyoctanoic acid side-chain via chain extension of a C4-precursor (4-hydroxybutyrate), and construction of the pyrrothine unit from cysteine via (HolA-D, F-H) prior to intact incorporation into thiomarinol (catalysed by TmlU). A series of thiomarinol-related and other minor metabolites have been isolated from wild-type and mutant strains. The results of these investigations are rationalised in terms of the overall biosynthetic pathway.
- External Organisation(s)
-
University of Bristol
University of Cambridge
University of Birmingham
Daiichi Sankyo Kabushiki-gaisha
- Type
- Article
- Journal
- Chemical science
- Volume
- 5
- Pages
- 397-402
- No. of pages
- 6
- ISSN
- 2041-6520
- Publication date
- 01.01.2014
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- General Chemistry
- Sustainable Development Goals
- SDG 14 - Life Below Water
- Electronic version(s)
-
https://doi.org/10.1039/c3sc52281d (Access:
Open)
