FRET-based and other fluorescent proteinase probes

authored by

Hai Yu Hu, Stefanie Gehrig, Gregor Reither, Devaraj Subramanian, Marcus A. Mall, Oliver Plettenburg, Carsten Schultz

Abstract

The continuous detection of enzyme activities and their application in medical diagnostics is one of the challenges in the translational sciences. Proteinases represent one of the largest groups of enzymes in the human genome and many diseases are based on malfunctions of proteolytic activity. Fluorescent sensors may shed light on regular and irregular proteinase activity in vitro and in vivo and provide a deeper insight into the function of these enzymes and their role in pathophysiological processes. The focus of this review is on Förster resonance energy transfer (FRET)-based proteinase sensors and reporters because these probes are most likely to provide quantitative data. The medical relevance of proteinases are discussed using lung diseases as a prominent example. Probe design and probe targeting are described and fluorescent probe development for disease-relevant proteinases, including matrix-metalloproteinases, cathepsins, caspases, and other selected proteinases, is reviewed.

External Organisation(s)

European Molecular Biology Laboratory
Sanofi-Aventis Deutschland GmbH
Heidelberg University

Type

Article

Journal

Biotechnology journal

Volume

9

Pages

266-281

No. of pages

16

ISSN

1860-6768

Publication date

04.02.2014

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Applied Microbiology and Biotechnology, Molecular Medicine

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.1002/biot.201300201 (Access: Closed)