Feruloyl esterases from Schizophyllum commune to treat food industry side-streams
- authored by
- Annabel Nieter, Sebastian Kelle, Diana Linke, Ralf G. Berger
- Abstract
Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.
- Organisation(s)
-
Institute of Food Chemistry
- Type
- Article
- Journal
- Bioresource technology
- Volume
- 220
- Pages
- 38-46
- No. of pages
- 9
- ISSN
- 0960-8524
- Publication date
- 12.08.2016
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Bioengineering, Environmental Engineering, Renewable Energy, Sustainability and the Environment, Waste Management and Disposal
- Sustainable Development Goals
- SDG 7 - Affordable and Clean Energy
- Electronic version(s)
-
https://doi.org/10.1016/j.biortech.2016.08.045 (Access:
Closed)
