Transreplication of a Tomato yellow leaf curl Thailand virus DNA-B and replication of a DNAß component by Tomato leaf curl Vietnam virus and Tomato yellow leaf curl Vietnam virus

authored by: R. Blawid, D. T. Van, E. Maiss
Abstract: The genomes of two tomato-infecting begomoviruses from Vietnam were cloned and sequenced. A new variant of Tomato leaf curl Vietnam virus (ToLCVV) consisting of a DNA-A component and associated with a DNAß molecule as well as an additional begomovirus tentatively named Tomato yellow leaf curl Vietnam virus (TYLCVV) consisting also of a DNA-A component were identified. To verify if monopartite viruses occurring in Vietnam and Thailand are able to transreplicate the DNA-B component of Tomato yellow leaf curl Thailand virus-[Asian Institute of Technology] (TYLCTHV-[AIT]) infectivity assays were performed via agroinoculation and mechanically. As result, the DNA-B component of TYLCTHV-[AIT] was transreplicated by different DNA-A components of viruses from Vietnam and Thailand in Nicotiana benthamiana and Solanum lycopersicum. Moreover, the TYLCTHV-[AIT] DNA-B component facilitated the mechanical transmission of monopartite viruses by rub-inoculation as well as by particle bombardment in N. benthamiana and tomato plants. Finally, defective DNAs ranging from 735 to 1457 nucleotides were generated in N. benthamiana from those combinations containing TYLCTHV-[AIT] DNA-B component.
Organisation(s): Phytomedicine Section
External Organisation(s): Research Institute of Fruits and Vegetables (RIFAV), Vietnam
Type: Article
Journal: Virus research
Volume: 136
Pages: 107-117
No. of pages: 11
ISSN: 0168-1702
Publication date: 09.2008
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Cancer Research, Virology, Infectious Diseases
Sustainable Development Goals: SDG 3 - Good Health and Well-being
Electronic version(s): https://doi.org/10.1016/j.virusres.2008.04.025 (Access: Closed)