Detection of feruloyl- and cinnamoyl esterases from basidiomycetes in the presence of interfering laccase

authored by

Paul Haase-Aschoff, Diana Linke, Ralf G. Berger

Abstract

Little is known on basidiomycete sources of feruloyl esterases (FAEs), although many wood-rotting representatives of these fungi typically grow on feruloyl-rich substrates. A major reason is that the almost ubiquitous presence of laccases interferes with the detection of FAE activity. Laccases polymerize the liberated ferulic acid (FA). in situ, thus detracting the product of enzymatic hydrolysis from its detection. A rapid HPLC-UV method was developed to detect the loss of FA, but also to quantify the hydrolysis of FA esters. The method allows at the same time to evaluate the substrate specificity of a FAE. Forty one basidiomycetes were tested for their FAE activities, and 25 out of the set were positive. The basidiomycetes hydrolyzing cinnamates with the highest conversion rates were. Auricularia auricula-judae and. Marasmius scorodonius. Moreover, a new FAE inducer, the nonionic detergent Tween 80, was found. This is the first comprehensive study on basidiomycete sources of FAEs.

Organisation(s)

Institute of Food Chemistry

Type

Article

Journal

Bioresource technology

Volume

130

Pages

231-238

No. of pages

8

ISSN

0960-8524

Publication date

20.12.2012

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Bioengineering, Environmental Engineering, Renewable Energy, Sustainability and the Environment, Waste Management and Disposal

Sustainable Development Goals

SDG 7 - Affordable and Clean Energy

Electronic version(s)

https://doi.org/10.1016/j.biortech.2012.12.039 (Access: Unknown)