Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone

authored by

Alejandra B. Omarini, Ina Plagemann, Silke Schimanski, Ulrich Krings, Ralf G. Berger

Abstract

Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast. ×. fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.

Organisation(s)

Institute of Food Chemistry

Type

Article

Journal

Bioresource technology

Volume

171

Pages

113-119

No. of pages

7

ISSN

0960-8524

Publication date

22.08.2014

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Bioengineering, Environmental Engineering, Renewable Energy, Sustainability and the Environment, Waste Management and Disposal

Sustainable Development Goals

SDG 7 - Affordable and Clean Energy

Electronic version(s)

https://doi.org/10.1016/j.biortech.2014.08.061 (Access: Closed)