Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone

verfasst von

Alejandra B. Omarini, Ina Plagemann, Silke Schimanski, Ulrich Krings, Ralf G. Berger

Abstract

Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast. ×. fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.

Organisationseinheit(en)

Institut für Lebensmittelchemie

Typ

Artikel

Journal

Bioresource technology

Band

171

Seiten

113-119

Anzahl der Seiten

7

ISSN

0960-8524

Publikationsdatum

22.08.2014

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Bioengineering, Environmental engineering, Erneuerbare Energien, Nachhaltigkeit und Umwelt, Abfallwirtschaft und -entsorgung

Ziele für nachhaltige Entwicklung

SDG 7 – Erschwingliche und saubere Energie

Elektronische Version(en)

https://doi.org/10.1016/j.biortech.2014.08.061 (Zugang: Geschlossen)