Applying Fiber Optical Methods for Toxicological Testing in Vitro

authored by: Holger K. Maerz, Rainer Buchholz, Frank Emmrich, Frank Finks, Clive L. Geddes, Lutz Pfeifer, Ferdinand Raabe, Thomas Scheper, Elizabeth Ulrich, Uwe Marx
Abstract: The new medical developments, e.g. immune therapy, patient orientated chemotherapy or even gene therapy, create a questionable doubt to the further requirement of animal tests. Instead the call for humanitarian reproductive in vitro models becomes increasingly louder. Pharmaceutical usage of in vitro has a long proven history. In cancer research and therapy, the effect of chemostatica in vitro in the so-called oncobiogram is being tested; but the assays do not always correlate with in vivo-like drug resistance and sensitivity. We developed a drug test system in vitro, feasible for therapeutic drug monitoring (TDM) by the combination of tissue cultivation in hollow fiber bioreactors (HFBR) and fiber optic sensors for monitoring the pharmaceutical effect. Using two fiber optic sensors - an optical oxygen sensor (MOPS) and a metabolism detecting Laserfluoroscope (LF), we were able to successfully monitor the biological status of tissue culture and the drug or toxic effects of in vitro pharmaceutical testing. Furthermore, we developed and patented a system for monitoring the effect of minor toxic compounds (VOC) which can induce Sick Building Syndrome (SBS).
Organisation(s): Institute of Technical Chemistry
External Organisation(s): Leipzig Heart Institute GmbH
Technische Universität Berlin
Innovative Optische Messtechnik GmbH
Leipzig University
Type: Conference contribution
Volume: 3603
Pages: 228-239
No. of pages: 12
Publication date: 21.04.1999
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Electronic, Optical and Magnetic Materials, Condensed Matter Physics, Computer Science Applications, Applied Mathematics, Electrical and Electronic Engineering
Sustainable Development Goals: SDG 3 - Good Health and Well-being
Electronic version(s): https://doi.org/10.1117/12.346746 (Access: Closed)