Applying Fiber Optical Methods for Toxicological Testing in Vitro

verfasst von

Holger K. Maerz, Rainer Buchholz, Frank Emmrich, Frank Finks, Clive L. Geddes, Lutz Pfeifer, Ferdinand Raabe, Thomas Scheper, Elizabeth Ulrich, Uwe Marx

Abstract

The new medical developments, e.g. immune therapy, patient orientated chemotherapy or even gene therapy, create a questionable doubt to the further requirement of animal tests. Instead the call for humanitarian reproductive in vitro models becomes increasingly louder. Pharmaceutical usage of in vitro has a long proven history. In cancer research and therapy, the effect of chemostatica in vitro in the so-called oncobiogram is being tested; but the assays do not always correlate with in vivo-like drug resistance and sensitivity. We developed a drug test system in vitro, feasible for therapeutic drug monitoring (TDM) by the combination of tissue cultivation in hollow fiber bioreactors (HFBR) and fiber optic sensors for monitoring the pharmaceutical effect. Using two fiber optic sensors - an optical oxygen sensor (MOPS) and a metabolism detecting Laserfluoroscope (LF), we were able to successfully monitor the biological status of tissue culture and the drug or toxic effects of in vitro pharmaceutical testing. Furthermore, we developed and patented a system for monitoring the effect of minor toxic compounds (VOC) which can induce Sick Building Syndrome (SBS).

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

Leipzig Heart Institute GmbH
Technische Universität Berlin
Innovative Optische Messtechnik GmbH
Universität Leipzig

Typ

Aufsatz in Konferenzband

Band

3603

Seiten

228-239

Anzahl der Seiten

12

Publikationsdatum

21.04.1999

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Elektronische, optische und magnetische Materialien, Physik der kondensierten Materie, Angewandte Informatik, Angewandte Mathematik, Elektrotechnik und Elektronik

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1117/12.346746 (Zugang: Geschlossen)