3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells

authored by

Vitalii V. Mutsenko, Vasilii V. Bazhenov, Olena Rogulska, Dmitriy N. Tarusin, Kathleen Schütz, Sophie Brüggemeier, Elke Gossla, Ashwini R. Akkineni, Heike Meißner, Anja Lode, Stephan Meschke, Andre Ehrlich, Slavica Petović, Rajko Martinović, Mirko Djurović, Allison L. Stelling, Sergey Nikulin, Sergey Rodin, Alexander Tonevitsky, Michael Gelinsky, Alexander Y. Petrenko, Birgit Glasmacher, Hermann Ehrlich

Abstract

The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7 days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.

Organisation(s)

Institute of Multiphase Processes

External Organisation(s)

TU Bergakademie Freiberg - University of Resources
Technische Universität Dresden
BromMarin GmbH
University of Montenegro
Duke University
Moscow Institute of Physics and Technology
P. Hertsen Moscow Oncology Research Institute
National Academy of Sciences of Ukraine
Institute for Problems of Cryobiology and Cryomedicine

Type

Article

Journal

International Journal of Biological Macromolecules

Volume

104

Pages

1966-1974

No. of pages

9

ISSN

0141-8130

Publication date

11.2017

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Structural Biology, Biochemistry, Molecular Biology, Economics and Econometrics, General Energy

Sustainable Development Goals

SDG 7 - Affordable and Clean Energy

Electronic version(s)

https://doi.org/10.1016/j.ijbiomac.2017.03.116 (Access: Closed)