3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells

verfasst von

Vitalii V. Mutsenko, Vasilii V. Bazhenov, Olena Rogulska, Dmitriy N. Tarusin, Kathleen Schütz, Sophie Brüggemeier, Elke Gossla, Ashwini R. Akkineni, Heike Meißner, Anja Lode, Stephan Meschke, Andre Ehrlich, Slavica Petović, Rajko Martinović, Mirko Djurović, Allison L. Stelling, Sergey Nikulin, Sergey Rodin, Alexander Tonevitsky, Michael Gelinsky, Alexander Y. Petrenko, Birgit Glasmacher, Hermann Ehrlich

Abstract

The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7 days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.

Organisationseinheit(en)

Institut für Mehrphasenprozesse

Externe Organisation(en)

Technische Universität Bergakademie Freiberg
Technische Universität Dresden
BromMarin GmbH
University of Montenegro
Duke University
Moscow Institute of Physics and Technology
P. Hertsen Moscow Oncology Research Institute
Nationale Akademie der Wissenschaften der Ukraine
Institute for Problems of Cryobiology and Cryomedicine

Typ

Artikel

Journal

International Journal of Biological Macromolecules

Band

104

Seiten

1966-1974

Anzahl der Seiten

9

ISSN

0141-8130

Publikationsdatum

11.2017

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Strukturelle Biologie, Biochemie, Molekularbiologie, Volkswirtschaftslehre und Ökonometrie, Allgemeine Energie

Ziele für nachhaltige Entwicklung

SDG 7 – Erschwingliche und saubere Energie

Elektronische Version(en)

https://doi.org/10.1016/j.ijbiomac.2017.03.116 (Zugang: Geschlossen)