Dengue-specific subviral nanoparticles

Design, creation and characterization

authored by

Niyati Khetarpal, Ankur Poddar, Satish K. Nemani, Nisha Dhar, Aravind Patil, Priyanka Negi, Ashiya Perween, Ramaswamy Viswanathan, Heinrich Lünsdorf, Poornima Tyagi, Rajendra Raut, Upasana Arora, Swatantra K. Jain, Ursula Rinas, Sathyamangalam Swaminathan, Navin Khanna

Abstract

Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.

Organisation(s)

Institute of Technical Chemistry

External Organisation(s)

International Centre for Genetic Engineering and Biotechnology
Helmholtz Centre for Infection Research (HZI)
Jamia Hamdard

Type

Article

Journal

Journal of nanobiotechnology

Volume

11

ISSN

1477-3155

Publication date

25.05.2013

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Bioengineering, Medicine (miscellaneous), Molecular Medicine, Biomedical Engineering, Applied Microbiology and Biotechnology, Pharmaceutical Science

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.1186/1477-3155-11-15 (Access: Open)