Dengue-specific subviral nanoparticles
Design, creation and characterization
- verfasst von
- Niyati Khetarpal, Ankur Poddar, Satish K. Nemani, Nisha Dhar, Aravind Patil, Priyanka Negi, Ashiya Perween, Ramaswamy Viswanathan, Heinrich Lünsdorf, Poornima Tyagi, Rajendra Raut, Upasana Arora, Swatantra K. Jain, Ursula Rinas, Sathyamangalam Swaminathan, Navin Khanna
- Abstract
Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
- Organisationseinheit(en)
-
Institut für Technische Chemie
- Externe Organisation(en)
-
International Centre for Genetic Engineering and Biotechnology
Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)
Jamia Hamdard
- Typ
- Artikel
- Journal
- Journal of nanobiotechnology
- Band
- 11
- ISSN
- 1477-3155
- Publikationsdatum
- 25.05.2013
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Bioengineering, Medizin (sonstige), Molekularmedizin, Biomedizintechnik, Angewandte Mikrobiologie und Biotechnologie, Pharmazeutische Wissenschaften
- Ziele für nachhaltige Entwicklung
- SDG 3 – Gute Gesundheit und Wohlergehen
- Elektronische Version(en)
-
https://doi.org/10.1186/1477-3155-11-15 (Zugang:
Offen)
