Violaxanthin cycle kinetics analysed in vivo with resonance Raman spectroscopy

authored by

Matthias Koch, Serge Zagermann, Ann Kathrin Kniggendorf, Merve Meinhardt-Wollweber, Bernhard Roth

Abstract

Measuring the kinetics of the violaxanthin cycle imposes an experimental challenge. Traditionally, carotenoid analysis was carried out laboriously with high-performance liquid chromatography. In this work, we present the first in vivo approach to directly measure the kinetics of the violaxanthin cycle, using resonance Raman spectroscopy in combination with baseline correction and principal component analysis. Applying the new approach allows measuring thousands of data points as opposed to the few possible with chemical analysis over the course of a violaxanthin cycle kinetics experiment. In vivo analysis of the violaxanthin cycle is necessary to fully understand adaptation kinetics to varying light conditions, the knowledge of which is especially important for assessing the stress tolerance of plants in the wake of the increasing climate change. Three experiments on the green alga Dunaliella salina were performed, featuring both the light-to-dark and dark-to-light transitions response of the algae.

Organisation(s)

Hannover Centre for Optical Technologies (HOT)

Type

Article

Journal

Journal of Raman spectroscopy

Volume

48

Pages

686-691

No. of pages

6

ISSN

0377-0486

Publication date

05.2017

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

General Materials Science, Spectroscopy

Sustainable Development Goals

SDG 13 - Climate Action

Electronic version(s)

https://doi.org/10.15488/1214 (Access: Open)
https://doi.org/10.1002/jrs.5102 (Access: Closed)