Selective cleavage of the HIV-1 TAR-RNA with a peptide-cyclen conjugate

authored by

Katrin Michaelis, Markus Kalesse

Abstract

Covalent linkage of the arginine-rich fragment of the Tat protein to 1,4,7,10-tetraazacyclododecane (cyclen) results in the selective cleavage of the TAR-RNA of HIV-1 (see picture; the biotin at the 5' end acts as a label for the subsequent analysis of the cleavage fragments). The cleavage occurs at room temperature and is diminished when Eu(III) ions are present - at a concentration of about 1/10 of the concentration of the peptide-cyclen conjugate. The pH dependence indicates that two ammonium ions are responsible for the cleavage reaction. The white arrows in the schematic diagram mark the cleavage sites in RNaseT1, and the black arrows the sites in the peptide- cyclen conjugate.

Organisation(s)

Institute of Organic Chemistry

Type

Article

Journal

Angewandte Chemie - International Edition

Volume

38

Pages

2243-2245

No. of pages

3

ISSN

1433-7851

Publication date

28.07.1999

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Catalysis, General Chemistry

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.1002/(SICI)1521-3773(19990802)38:15<2243::AID-ANIE2243>3.0.CO;2-V (Access: Closed)