Selective cleavage of the HIV-1 TAR-RNA with a peptide-cyclen conjugate
- verfasst von
- Katrin Michaelis, Markus Kalesse
- Abstract
Covalent linkage of the arginine-rich fragment of the Tat protein to 1,4,7,10-tetraazacyclododecane (cyclen) results in the selective cleavage of the TAR-RNA of HIV-1 (see picture; the biotin at the 5' end acts as a label for the subsequent analysis of the cleavage fragments). The cleavage occurs at room temperature and is diminished when Eu(III) ions are present - at a concentration of about 1/10 of the concentration of the peptide-cyclen conjugate. The pH dependence indicates that two ammonium ions are responsible for the cleavage reaction. The white arrows in the schematic diagram mark the cleavage sites in RNaseT1, and the black arrows the sites in the peptide- cyclen conjugate.
- Organisationseinheit(en)
-
Institut für Organische Chemie
- Typ
- Artikel
- Journal
- Angewandte Chemie - International Edition
- Band
- 38
- Seiten
- 2243-2245
- Anzahl der Seiten
- 3
- ISSN
- 1433-7851
- Publikationsdatum
- 28.07.1999
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Katalyse, Allgemeine Chemie
- Ziele für nachhaltige Entwicklung
- SDG 3 – Gute Gesundheit und Wohlergehen
- Elektronische Version(en)
-
https://doi.org/10.1002/(SICI)1521-3773(19990802)38:15<2243::AID-ANIE2243>3.0.CO;2-V (Zugang:
Geschlossen)
