Xeno-free in vitro cultivation and osteogenic differentiation of hAD-MSCs on resorbable 3D printed RESOMER®

verfasst von

Marline Kirsch, Annabelle Christin Herder, Cécile Boudot, Andreas Karau, Jessica Rach, Wiebke Handke, Axel Seltsam, Thomas Scheper, Antonina Lavrentieva

Abstract

The development of alloplastic resorbable materials can revolutionize the field of implantation technology in regenerative medicine. Additional opportunities to colonize the three-dimensionally (3D) printed constructs with the patient's own cells prior to implantation can improve the regeneration process but requires optimization of cultivation protocols. Human platelet lysate (hPL) has already proven to be a suitable replacement for fetal calf serum (FCS) in 2D and 3D cell cultures. In this study, we investigated the in vitro biocompatibility of the printed RESOMER® Filament LG D1.75 materials as well as the osteogenic differentiation of human mesenchymal stem cells (hMSCs) cultivated on 3D printed constructs under the influence of different medium supplements (FCS, human serum (HS) and hPL). Additionally, the in vitro degradation of the material was studied over six months. We demonstrated that LG D1.75 is biocompatible and has no in vitro cytotoxic effects on hMSCs. Furthermore, hMSCs grown on the constructs could be differentiated into osteoblasts, especially supported by supplementation with hPL. Over six months under physiological in vitro conditions, a distinct degradation was observed, which, however, had no influence on the biocompatibility of the material. Thus, the overall suitability of the material LG D1.75 to produce 3D printed, resorbable bone implants and the promising use of hPL in the xeno-free cultivation of human MSCs on such implants for autologous transplantation have been demonstrated.

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

Evonik Nutrition and Care GmbH
Deutsches Rotes Kreuz e. V. (DRK)

Typ

Artikel

Journal

MATERIALS

Band

13

Anzahl der Seiten

17

ISSN

1996-1944

Publikationsdatum

31.07.2020

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Werkstoffwissenschaften (insg.)

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.3390/ma13153399 (Zugang: Offen)