Bioactive Phenolic Compounds from Lingonberry (Vaccinium vitis-idaea L.): Extraction, Chemical Characterization, Fractionation and Cellular Antioxidant Activity

verfasst von

Tina Kostka, Johanna Josefine Ostberg-Potthoff, Joachim Stärke, Claudia Guigas, Seiichi Matsugo, Valentin Mirčeski, Leon Stojanov, Sanja Kostadinović Veličkovska, Peter Winterhalter, Tuba Esatbeyoglu

Abstract

Lingonberries contain high contents of bioactive compounds such as chlorogenic acids and anthocyanins. In addition to radical scavenging and antioxidant activities, these compounds can protect cells from DNA damage. For this reason, lingonberries might be well suited for nutraceuticals or natural biomedicines. To assess these applications, the present study characterized and identified the most effective extract, only consisting of anthocyanins, copigments or a mixture of both, obtained from a lingonberry juice concentrate. An extract was generated by using a XAD-7 column followed by fractionation into anthocyanins and copigments using adsorptive membrane chromatography. After identification of main polyphenols by HPLC–photodiode array–electrospray ionization–tandem mass spectrometry, free radical scavenging activity was analyzed by electron spin resonance spectroscopy using 2,2-diphenyl-1-picrylhydrazyl and galvinoxyl radicals. Furthermore, cyclic voltammetry analyses and the Trolox equivalent antioxidant capacity (TEAC) assay were applied. Finally, the reactive oxygen species (ROS) reducing effects of the lingonberry extract and its fractions were evaluated in HepG2 cells. While the combination of anthocyanins and copigments possessed the highest antioxidant activities, all samples (XAD-7 extract, anthocyanin and copigment fraction) protected cells from oxidative stress. Thus, synergistic effects between phenolic compounds may be responsible for the high antioxidant potential of lingonberries, enabling their use as nutraceuticals.

Organisationseinheit(en)

Molekulare Lebensmittelchemie und -entwicklung
Institut für Lebensmittelwissenschaft und Humanernährung

Externe Organisation(en)

Max Rubner-Institut, Bundesforschungsinstitut für Ernährung und Lebensmittel
Technische Universität Braunschweig
Kanazawa University
Uniwersytet Łódzki
Ss. Cyril and Methodius University
Goce Delchev University (UGD)

Typ

Artikel

Journal

Antioxidants

Band

11

Publikationsdatum

26.02.2022

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Molekularbiologie, Biochemie, Physiologie, Klinische Biochemie, Zellbiologie

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.3390/antiox11030467 (Zugang: Offen)