In vitro toxicological nanoparticle studies under flow exposure

verfasst von: Franziska Sambale, Frank Stahl, Detlef Bahnemann, Thomas Scheper
Abstract: The use of nanoparticles is becoming increasingly common in industry and everyday objects. Thus, extensive risk management concerning the potential health risk of nanoparticles is important. Currently, in vitro nanoparticle testing is mainly performed under static culture conditions without any shear stress. However, shear stress is an important biomechanical parameter. Therefore, in this study, a defined physiological flow to different mammalian cell lines such as A549 cells and NIH-3T3 cells has been applied. The effects of zinc oxide and titanium dioxide nanoparticles (TiO₂-NP), respectively, were investigated under both static and dynamic conditions. Cell viability, cell morphology, and adhesion were proven and compared to the static cell culture. Flow exposure had an impact on the cellular morphology of the cells. NIH-3T3 cells were elongated in the direction of flow and A549 cells exhibited vesicles inside the cells. Zinc oxide nanoparticles reduced the cell viability in the static and in the dynamic culture; however, the dynamic cultures were more sensitive. In the static culture and in the dynamic culture, TiO₂-NP did not affect cell viability. In conclusion, dynamic culture conditions are important for further in vitro investigations and provide more relevant results than static culture conditions.
Organisationseinheit(en): Institut für Technische Chemie
Externe Organisation(en): Staatliche Universität Sankt Petersburg
Typ: Artikel
Journal: Journal of nanoparticle research
Band: 17
ISSN: 1388-0764
Publikationsdatum: 11.07.2015
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Bioengineering, Chemie (insg.), Atom- und Molekularphysik sowie Optik, Modellierung und Simulation, Werkstoffwissenschaften (insg.), Physik der kondensierten Materie
Ziele für nachhaltige Entwicklung: SDG 3 – Gute Gesundheit und Wohlergehen
Elektronische Version(en): https://doi.org/10.1007/s11051-015-3106-2 (Zugang: Geschlossen)