Longitudinal Claudin Gene Expression Analyses in Canine Mammary Tissues and Thereof Derived Primary Cultures and Cell Lines

verfasst von

Susanne C Hammer, Annegret Becker, Katja Rateitschak, Annika Mohr, Florenza Lüder Ripoli, Silvia Hennecke, Johannes Junginger, Marion Hewicker-Trautwein, Bertram Brenig, Anaclet Ngezahayo, Ingo Nolte, Hugo Murua Escobar

Abstract

Human and canine mammary tumours show partial claudin expression deregulations. Further, claudins have been used for directed therapeutic approaches. However, the development of claudin targeting approaches requires stable claudin expressing cell lines. This study reports the establishment and characterisation of canine mammary tissue derived cell lines, analysing longitudinally the claudin-1, -3, -4 and -7 expressions in original tissue samples, primary cultures and developed cell lines. Primary cultures were derived from 17 canine mammary tissues: healthy, lobular hyperplasia, simple adenoma, complex adenoma, simple tubular carcinoma, complex carcinoma, carcinoma arising in a benign mixed tumour and benign mixed tissue. Cultivation was performed, if possible, until passage 30. Claudin mRNA and protein expressions were analysed by PCR, QuantiGene Plex Assay, immunocytochemistry and immunofluorescence. Further, cytokeratin expression was analysed immunocytochemically. Cultivation resulted in 11 established cell lines, eight showing epithelial character. In five of the early passages the claudin expressions decreased compared to the original tissues. In general, claudin expressions were diminished during cultivation. Three cell lines kept longitudinally claudin, as well as epithelial marker expressions, representing valuable tools for the development of claudin targeted anti-tumour therapies.

Organisationseinheit(en)

Institut für Zellbiologie und Biophysik

Externe Organisation(en)

Stiftung Tierärztliche Hochschule Hannover
Universität Greifswald
Georg-August-Universität Göttingen
Zentrum für Systemische Neurowissenschaften Hannover (ZSN)

Typ

Artikel

Journal

International Journal of Molecular Sciences

Band

17

ISSN

1422-0067

Publikationsdatum

29.09.2016

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Molekularbiologie, Spektroskopie, Katalyse, Anorganische Chemie, Angewandte Informatik, Physikalische und Theoretische Chemie, Organische Chemie

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.3390/ijms17101655 (Zugang: Offen)