Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells

verfasst von: Douglass Wu, Guo Wen Xing, Michael A. Poles, Amir Horowitz, Yuki Kinjo, Barbara Sullivan, Vera Bodmer-Narkevitch, Oliver Plettenburg, Mitchell Kronenberg, Moriya Tsuji, David D. Ho, Chi Huey Wong
Abstract: The CD1 family of proteins binds self and foreign glycolipids for presentation to CD1-restricted T cells. To identify previously uncharacterized active CD1 ligands, especially those of microbial origin, numerous glycolipids were synthesized and tested for their ability to stimulate mouse and human natural killer T (NKT) cells. They included analogs of the well known NKT cell agonist α-galactosyl ceramide (α-GalCer), bacterial glycolipids, and variations of the self-glycolipid, sulfatide. Bacterial glycolipids, α-galacturonosyl-ceramides from Sphingomonas wittichii, although structurally similar to α-GalCer, have significant differences in the sugar head group as well as the ceramide portion. The Sphingomonas glycosphingolipids (GSLs) and sulfatide variants were shown to activate human NKT cells as measured by IL-4 and IFN-γ secretion. Moreover, CD1d-dimer staining revealed human NKT cell reactivity toward these GSLs and to the sulfatides in a fashion comparable with α-GalCer. Because α-GalCer is a marine-sponge-derived ligand, our study here shows that bacterium-derived antigens are also able to stimulate mouse and human NKT cells.
Externe Organisation(en): The Scripps Research Translational Institute
Rockefeller University
New York University (NYU)
La Jolla Institute for Allergy and Immunology
Typ: Artikel
Journal: Proceedings of the National Academy of Sciences of the United States of America
Band: 102
Seiten: 1351-1356
Anzahl der Seiten: 6
ISSN: 0027-8424
Publikationsdatum: 01.02.2005
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Allgemein
Ziele für nachhaltige Entwicklung: SDG 14 – Lebensraum Wasser
Elektronische Version(en): https://doi.org/10.1073/pnas.0408696102 (Zugang: Offen)