Engineered Tobacco mosaic virus mutants with distinct physical characteristics in planta and enhanced metallization properties

verfasst von

Anan Kadri, Edgar Maiß, Nadja Amsharov, Alexander M. Bittner, Sinan Balci, Klaus Kern, Holger Jeske, Christina Wege

Abstract

Tobacco mosaic virus mutants were engineered to alter either the stability or surface chemistry of the virion: within the coat protein, glutamic acid was exchanged for glutamine in a buried portion to enhance the inter-subunit binding stability (E50Q), or a hexahistidine tract was fused to the surface-exposed carboxy terminus of the coat protein (6xHis). Both mutant viruses were expected to possess specific metal ion affinities. They accumulated to high titers in plants, induced distinct phenotypes, and their physical properties during purification differed from each other and from wild type (wt) virus. Whereas 6xHis and wt virions contained RNA, the majority of E50Q protein assembled essentially without RNA into rods which frequently exceeded 2 μm in length. Electroless deposition of nickel metallized the outer surface of 6xHis virions, but the central channel of E50Q rods, with significantly more nanowires of increased length in comparison to those formed in wtTMV.

Organisationseinheit(en)

Institut für Gartenbauliche Produktionssysteme

Externe Organisation(en)

Universität Stuttgart
Max-Planck-Institut für Festkörperforschung

Typ

Artikel

Journal

Virus research

Band

157

Seiten

35-46

Anzahl der Seiten

12

ISSN

0168-1702

Publikationsdatum

08.02.2011

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Krebsforschung, Virologie, Infektionskrankheiten

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1016/j.virusres.2011.01.014 (Zugang: Geschlossen)