Bacterial inclusion bodies are industrially exploitable amyloids

verfasst von

Ario De Marco, Neus Ferrer-Miralles, Elena Garcia-Fruitós, Anna Mitraki, Spela Peternel, Ursula Rinas, Mauricio A. Trujillo-Roldán, Norma A. Valdez-Cruz, Esther Vázquez, Antonio Villaverde

Abstract

Understanding the structure, functionalities and biology of functional amyloids is an issue of emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria during protein production processes, have emerged as unanticipated, highly tunable models for the scrutiny of the physiology and architecture of functional amyloids. Based on an amyloidal skeleton combined with varying amounts of native or native-like protein forms, bacterial inclusion bodies exhibit an unusual arrangement that confers mechanical stability, biological activity and conditional protein release, being thus exploitable as versatile biomaterials. The applicability of inclusion bodies in biotechnology as enriched sources of protein and reusable catalysts, and in biomedicine as biocompatible topographies, nanopills or mimetics of endocrine secretory granules has been largely validated. Beyond these uses, the dissection of how recombinant bacteria manage the aggregation of functional protein species into structures of highly variable complexity offers insights about unsuspected connections between protein quality (conformational status compatible with functionality) and cell physiology.

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

University of Nova Gorica
Universidad Autónoma de Barcelona (UAB)
Centros de Investigacion Biomedica en Red - CIBER
IRTA - Institute of Agrifood Research and Technology
University of Crete
Lupinica
Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)
Universidad Nacional Autónoma de México (UNAM)

Typ

Übersichtsarbeit

Journal

FEMS Microbiology Reviews

Band

43

Seiten

53-72

Anzahl der Seiten

20

ISSN

0168-6445

Publikationsdatum

01.2019

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Mikrobiologie, Infektionskrankheiten

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1093/femsre/fuy038 (Zugang: Offen)