3D models of the hematopoietic stem cell niche under steady-state and active conditions

verfasst von

Lisa Rödling, Ivo Schwedhelm, Saskia Kraus, Karen Bieback, Jan Hansmann, Cornelia Lee-Thedieck

Abstract

Hematopoietic stem cells (HSCs) in the bone marrow are able to differentiate into all types of blood cells and supply the organism each day with billions of fresh cells. They are applied to cure hematological diseases such as leukemia. The clinical need for HSCs is high and there is a demand for being able to control and multiply HSCs in vitro. The hematopoietic system is highly proliferative and thus sensitive to anti-proliferative drugs such as chemotherapeutics. For many of these drugs suppression of the hematopoietic system is the dose-limiting toxicity. Therefore, biomimetic 3D models of the HSC niche that allow to control HSC behavior in vitro and to test drugs in a human setting are relevant for the clinics and pharmacology. Here, we describe a perfused 3D bone marrow analog that allows mimicking the HSC niche under steady-state and activated conditions that favor either HSC maintenance or differentiation, respectively, and allows for drug testing.

Organisationseinheit(en)

Abteilung Zellbiologie

Typ

Artikel

Journal

Scientific reports

Band

7

ISSN

2045-2322

Publikationsdatum

12.2017

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1038/s41598-017-04808-0 (Zugang: Offen)