Enhancing chemotherapeutic efficacy

Niosome-encapsulated Dox-Cis with MUC-1 aptamer

verfasst von

Firat Baris Barlas, Bilge Olceroglu, Didem Ag Seleci, Zinar Pinar Gumus, Pinar Siyah, Meriam Dabbek, Georg Garnweitne, Frank Stahl, Thomas Scheper, Suna Timur

Abstract

Background: Cancer remains a formidable global health challenge, currently affecting nearly 20 million individuals worldwide. Due to the absence of universally effective treatments, ongoing research explores diverse strategies to combat this disease. Recent efforts have concentrated on developing combined drug regimens and targeted therapeutic approaches. Objective: This study aimed to investigate the anticancer efficacy of a conjugated drug system, consisting of doxorubicin and cisplatin (Dox-Cis), encapsulated within niosomes and modified with MUC-1 aptamers to enhance biocompatibility and target specific cancer cells. Methods: The chemical structure of the Dox-Cis conjugate was characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (LC-Q-TOF/MS). The zeta potential and morphological parameters of the niosomal vesicles were determined through Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM). In vitro assessments of cell viability and apoptosis were conducted on MUC-1 positive HeLa cells and MUC-1 negative U87 cells. Results: The findings confirmed the successful conjugation of Dox and Cis within the niosomes. The Nio/Dox-Cis/MUC-1 formulation demonstrated enhanced efficacy compared to the individual drugs and their unencapsulated combination in both cell lines. Notably, the Nio/Dox-Cis/MUC-1 formulation exhibited greater effectiveness on HeLa cells (38.503 ± 1.407) than on U87 cells (46.653 ± 1.297). Conclusion: The study underscores the potential of the Dox-Cis conjugate as a promising strategy for cancer treatment, particularly through platforms that facilitate targeted drug delivery to cancer cells. This targeted approach could lead to more effective and personalized cancer therapies.

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

İstanbul Üniversitesi - Cerrahpaşa
Technische Universität Braunschweig
Ege University
Bahcesehir University

Typ

Artikel

Journal

Cancer medicine

Band

13

Anzahl der Seiten

10

ISSN

2045-7634

Publikationsdatum

09.08.2024

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Onkologie, Radiologie, Nuklearmedizin und Bildgebung, Krebsforschung

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1002/cam4.70079 (Zugang: Offen)