Microbial community analysis of deeply buried marine sediments of the New Jersey shallow shelf (IODP Expedition 313)

verfasst von

Anja Breuker, Susanne Stadler, Axel Schippers

Abstract

The investigated deeply buried marine sediments of the shallow shelf off New Jersey, USA, are characterized by low organic carbon content and total cell counts of < 10⁷ cells per mL sediment. The qPCR data for Bacteria and Archaea were in the same orders of magnitude as the total cell counts. Archaea and Bacteria occurred in similar 16S rRNA gene copy numbers in the upper part of the sediments, but Bacteria dominated in the lowermost part of the analyzed sediment cores down to a maximum analyzed depth of c. 50 meters below seafloor (mbsf). The bacterial candidate division JS1 and the classes Anaerolineae and Caldinilineae of the Chloroflexi were almost as highly abundant as the total Bacteria. Similarly high dsrA gene copy numbers were found for sulfate reducers. The abundance of the Fe(III) and Mn(IV) reducers comprising Geobacteraceae in the upper c. 15 mbsf correlated with concentrations of manganese and iron in the pore water. The isolated 16S rRNA gene sequences of Archaea in clone libraries could be allocated to the phyla Thaumarchaeota, Euryarchaeota, and Crenarchaeota with 1%, 14%, and 85%, respectively. The typical deep subsurface sediment-associated groups MBG-B, MBG-D, MCG, and SAGMEG were represented in the sediment community. MCG was the dominant group with a high diversity of the isolated 16S rRNA gene sequences.

Externe Organisation(en)

Bundesanstalt für Geowissenschaften und Rohstoffe (BGR)

Typ

Artikel

Journal

FEMS microbiology ecology

Band

85

Seiten

578-592

Anzahl der Seiten

15

ISSN

0168-6496

Publikationsdatum

01.09.2013

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Mikrobiologie, Ökologie, Angewandte Mikrobiologie und Biotechnologie

Ziele für nachhaltige Entwicklung

SDG 14 – Lebensraum Wasser

Elektronische Version(en)

https://doi.org/10.1111/1574-6941.12146 (Zugang: Offen)