Evaluation of stem cell marker gene expression in canine prostate carcinoma- and prostate cyst-derived cell lines
- verfasst von
- Mohammed Moulay, Wen Liu, Saskia Willenbrock, Katharina Anna Sterenczak, Regina Carlson, Anaclet Ngezahayo, Hugo Murua Escobar, Ingo Nolte
- Abstract
BACKGROUND/AIM: In human prostate cancer cells with a stem cell-like character (cancer stem cells, CSC) are considered to play a major role in disease development, progression and relapse. Aim of the study was to evaluate if similar cells are present and active in canine prostate cancer providing a naturally-occurring mammalian model for the development of therapeutic approaches targeting CSC.
MATERIALS AND METHODS: Stem cell marker expression of CD133, CD44, C-KIT, CD34, ITGA6, OCT4, DDX5 and MELK in canine prostate carcinomas and prostate cyst cell lines were screened by Polymerase Chain Reaction (PCR), quantitative Polymerase Chain Reaction (qPCR) and partially analysed by flow cytometry.
RESULTS: Marker analyses by PCR and qPCR, revealed a complex expression pattern for the analysed marker genes, providing a characteristic marker pattern for the studied cell lines. Thereby CD44, CD133, ITGA6 and DDX5 showed the most prominent expression in the analysed cell lines.
CONCLUSION: The results revealed a characteristic stem cell marker expression in the analysed cell lines, indicating the presence of CSC in canine prostate cancer.
- Organisationseinheit(en)
-
Abteilung Zellphysiologie und Biophysik
- Externe Organisation(en)
-
Stiftung Tierärztliche Hochschule Hannover
- Typ
- Artikel
- Journal
- Anticancer research
- Band
- 33
- Seiten
- 5421-31
- Anzahl der Seiten
- 11
- ISSN
- 0250-7005
- Publikationsdatum
- 12.2013
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- Ziele für nachhaltige Entwicklung
- SDG 3 – Gute Gesundheit und Wohlergehen
- Elektronische Version(en)
-
https://doi.org/doi: 10.1016/j.yexcr.2013.03.016 (Zugang:
Eingeschränkt)
