Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering

verfasst von

M. Granados, L. Morticelli, S. Andriopoulou, P. Kalozoumis, M. Pflaum, P. Iablonskii, Birgit Glasmacher, M. Harder, Jan Hegermann, C. Wrede, I. Tudorache, S. Cebotari, A. Hilfiker, A. Haverich, Sotirios Korossis

Abstract

Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colony-forming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IV on the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.

Organisationseinheit(en)

Institut für Mehrphasenprozesse

Externe Organisation(en)

Medizinische Hochschule Hannover (MHH)
Leibniz Forschungslaboratorien für Biotechnologie und künstliche Organe (LEBAO)
Corlife OHG

Typ

Artikel

Journal

Journal of Cardiovascular Translational Research

Band

10

Seiten

374-390

Anzahl der Seiten

17

ISSN

1937-5387

Publikationsdatum

08.2017

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Molekularmedizin, Genetik, Pharmazeutische Wissenschaften, Kardiologie und kardiovaskuläre Medizin, Genetik (klinisch)

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1007/s12265-017-9747-z (Zugang: Geschlossen)