Proteomic and physiological responses of the halophyte Cakile maritima to moderate salinity at the germinative and vegetative stages

verfasst von

Ahmed Debez, Hans Peter Braun, Andreas Pich, Wael Taamalli, Hans Werner Koyro, Chedly Abdelly, Bernhard Huchzermeyer

Abstract

Responses of the halophyte Cakile maritima to moderate salinity were addressed at germination and vegetative stages by bringing together proteomics and eco-physiological approaches. 75. mM NaCl-salinity delayed significantly the germination process and decreased slightly the seed germination percentage compared to salt-free conditions. Monitoring the proteome profile between 0. h and 120. h after seed sowing revealed a delay in the degradation of seed storage proteins when germination took place under salinity, which may explain the slower germination rate observed. Of the sixty-seven proteins identified by mass spectrometry, several proteins involved in glycolysis, amino acid metabolism, photosynthesis, and protein folding showed significantly increased abundance during germination. This pattern was less pronounced under salinity. At the vegetative stage, 100. mM NaCl-salinity stimulated significantly the plant growth, which was sustained by enhanced leaf expansion, water content, and photosynthetic activity. Comparative proteome analyses of leaf tissue revealed 44 proteins with different abundance changes, most of which being involved in energy metabolism. A specific set of proteins predominantly involved in photosynthesis and respiration showed significantly higher abundance in salt-treated plants. Altogether, combining proteomics with eco-physiological tools provides valuable information, which contributes to improve our understanding in the salt-response of this halophyte during its life cycle.

Organisationseinheit(en)

Institut für Pflanzengenetik

Externe Organisation(en)

Centre de Biotechnologie de Borj Cédria (CBBC)
Medizinische Hochschule Hannover (MHH)
Justus-Liebig-Universität Gießen

Typ

Artikel

Journal

Journal of proteomics

Band

75

Seiten

5667-5694

Anzahl der Seiten

28

ISSN

1874-3919

Publikationsdatum

29.08.2012

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biophysik, Biochemie

Ziele für nachhaltige Entwicklung

SDG 15 – Lebensraum Land

Elektronische Version(en)

https://doi.org/10.1016/j.jprot.2012.08.012 (Zugang: Unbekannt)