Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India

verfasst von

Elangovan Dilipan, Jutta Papenbrock, Thirunavakkarasu Thangaradjou

Abstract

In India 14 seagrass species can be found with monospecific genera (Enhalus, Thalassia and Syringodium), Cymodocea with two species and Halophila and Halodule represented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of the Halophila and Halodule complex still has to be tested before recommending these primers as an identification tool for Indian seagrasses.

Organisationseinheit(en)

Institut für Botanik

Externe Organisation(en)

Annamalai University

Typ

Artikel

Journal

Journal of the Marine Biological Association of the United Kingdom

Band

97

Seiten

1307-1315

Anzahl der Seiten

9

ISSN

0025-3154

Publikationsdatum

01.09.2017

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Aquatische Wissenschaften

Ziele für nachhaltige Entwicklung

SDG 14 – Lebensraum Wasser

Elektronische Version(en)

https://doi.org/10.1017/S0025315416000631 (Zugang: Unbekannt)