Development of living cell microarrays using non-contactmicropipette printing

verfasst von

Rebecca Jonczyk, Suna Timur, Thomas Scheper, Frank Stahl

Abstract

During the last 30 years cellular screening systems were unidirectional developed towards high throughput applications on single cell level. We developed living cell microarrays, which provide an in vivo-like microenvironment for an advanced method to measure cellular response to external stimuli. To print living cells on glass slides, the classic microarray equipment, which involves printer and scanner, was fully transferred to suspensions of living cells. The microarray production was optimized using a contact-free spotting procedure in order to enhanced cell adhesion and growth rates. The printed model cells, A-549 (lung cancer cell line), were analyzed with conventional cell staining assays like DAPI (cell nuclei staining), calcein acetoxymethyl ester (viable cell staining), and CellTiter-Blue^® Cell Viability Assay. After optimization, a reproducible (spot-to-spot variation:±8.6 cells) printing method for small living cell amounts (1200cells and fewer) was established that achieved cell viabilities of up to 88% for ≥0.6μL and good proliferation characteristics. Hence, this method could be advantageous for use in biomedical and diagnostic applications.

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

Ege University

Typ

Artikel

Journal

Journal of biotechnology

Band

217

Seiten

109-111

Anzahl der Seiten

3

ISSN

0168-1656

Publikationsdatum

19.11.2015

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biotechnologie, Bioengineering, Angewandte Mikrobiologie und Biotechnologie

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1016/j.jbiotec.2015.11.013 (Zugang: Geschlossen)