Hitherto unknown [Fe-Fe]-hydrogenase gene diversity in anaerobes and anoxic enrichments from a moderately acidic fen

verfasst von

Oliver Schmidt, Harold L. Drake, Marcus A. Horn

Abstract

Newly designed primers for [Fe-Fe]-hydrogenases indicated that (i) fermenters, acetogens, and undefined species in a fen harbor hitherto unknown hydrogenases and (ii) Clostridium- and Thermosinus-related primary fermenters, as well as secondary fermenters related to sulfate or iron reducers might be responsible for hydrogen production in the fen. Comparative analysis of [Fe-Fe]-hydrogenase and 16S rRNA gene-based phylogenies indicated the presence of homologous multiple hydrogenases per organism and inconsistencies between 16S rRNA gene- and [Fe-Fe]-hydrogenase-based phylogenies, necessitating appropriate qualification of [Fe-Fe]-hydrogenase gene data for diversity analyses.

Externe Organisation(en)

Universität Bayreuth

Typ

Artikel

Journal

Applied and Environmental Microbiology

Band

76

Seiten

2027-2031

Anzahl der Seiten

5

ISSN

0099-2240

Publikationsdatum

03.2010

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biotechnologie, Lebensmittelwissenschaften, Angewandte Mikrobiologie und Biotechnologie, Ökologie

Ziele für nachhaltige Entwicklung

SDG 7 – Erschwingliche und saubere Energie

Elektronische Version(en)

https://doi.org/10.1128/AEM.02895-09 (Zugang: Offen)
https://doi.org/10.15488/15921 (Zugang: Offen)