Possibilities and efficiency of MSC co-transfection for gene therapy

verfasst von

Sina Christoffers, Lisa Seiler, Elena Wiebe, Cornelia Blume

Abstract

Mesenchymal stem/stromal cells (MSCs) are not only capable of self-renewal, trans-differentiation, homing to damaged tissue sites and immunomodulation by secretion of trophic factors but are also easy to isolate and expand. Because of these characteristics, they are used in numerous clinical trials for cell therapy including immune and neurological disorders, diabetes, bone and cartilage diseases and myocardial infarction. However, not all trials have successful outcomes, due to unfavourable microenvironmental factors and the heterogenous nature of MSCs. Therefore, genetic manipulation of MSCs can increase their prospect. Currently, most studies focus on single transfection with one gene. Even though the introduction of more than one gene increases the complexity, it also increases the effectivity as different mechanism are triggered, leading to a synergistic effect. In this review we focus on the methodology and efficiency of co-transfection, as well as the opportunities and pitfalls of these genetically engineered cells for therapy. Graphical abstract: (Figure presented.)

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

Exzellenzcluster Hearing4all

Typ

Übersichtsarbeit

Journal

Stem Cell Research and Therapy

Band

15

Anzahl der Seiten

17

ISSN

1757-6512

Publikationsdatum

23.05.2024

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Medizin (sonstige), Molekularmedizin, Biochemie, Genetik und Molekularbiologie (sonstige), Zellbiologie

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1186/s13287-024-03757-6 (Zugang: Offen)