Antifungal Susceptibility Testing of Aspergillus Niger on Silicon Microwells by Intensity-Based Reflectometric Interference Spectroscopy

verfasst von

Christopher Heuer, Heidi Leonard, Nadav Nitzan, Ariella Lavy-Alperovitch, Naama Massad-Ivanir, Thomas Scheper, Ester Segal

Abstract

There is a demonstrated and paramount need for rapid, reliable infectious disease diagnostics, particularly those for invasive fungal infections. Current clinical determinations for an appropriate antifungal therapy can take up to 3 days using current antifungal susceptibility testing methods, a time-to-readout that can prove detrimental for immunocompromised patients and promote the spread of antifungal resistant pathogens. Herein, we demonstrate the application of intensity-based reflectometric interference spectroscopic measurements (termed iPRISM) on microstructured silicon sensors for use as a rapid, phenotypic antifungal susceptibility test. This diagnostic platform optically tracks morphological changes of fungi corresponding to conidia growth and hyphal colonization at a solid-liquid interface in real time. Using Aspergillus niger as a model fungal pathogen, we can determine the minimal inhibitory concentration of clinically relevant antifungals within 12 h. This assay allows for expedited detection of fungal growth and provides a label-free alternative to broth microdilution and agar diffusion methods, with the potential to be used for point-of-care diagnostics.

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

Technion-Israel Institute of Technology

Typ

Artikel

Journal

ACS infectious diseases

Band

6

Seiten

2560-2566

Anzahl der Seiten

7

ISSN

2373-8227

Publikationsdatum

09.10.2020

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Infektionskrankheiten

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1021/acsinfecdis.0c00234 (Zugang: Offen)