Transcriptomics does not show adverse effects of β-carotene in A/J mice exposed to smoke for 2 weeks

verfasst von

Emmanuelle Kuntz, Jürgen Borlak, Georges Riss, Claude Pierre Aebischer, Heinrich Bachmann, Nicole Seifert, Petra Buchwald Hunziker, Dörte Sölle, Willi Hunziker, Regina Goralczyk, Karin Wertz

Abstract

β-Carotene (βC) supplementation in smokers was unexpectedly associated with increased incidence of lung cancer versus smoking alone. We performed a study in A/J mice to explore possible βC/cigarette smoke (CS) interactions potentially influencing lung cancer risk in smokers. A/J mice received a diet containing 120 or 600 ppm βC for six weeks, and exposed to mainstream CS (140 mg total suspended particulates/m³) during the last two weeks. Lung transcriptomics analysis revealed that CS induced drug metabolism, oxidative stress, extracellular matrix (ECM) degradation, inflammation markers, and apoptosis. βC reduced CS-induced inflammation markers and ECM degradation. βC modulated the CS effect on apoptosis without a clear pro- or anti-apoptotic trend. βC alone induced only minor changes of gene expression. In conclusion, βC/CS interactions caused gene regulations in lungs. CS was the main effector. The gene regulations overall did not indicate that βC exacerbated CS effects. Dose-dependency of βC effects was minor and not detectable by genome-wide data mining.

Externe Organisation(en)

DSM Food Specialties
RCC Ltd.
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin (ITEM)
Herbonis AG
Frimorfo

Typ

Artikel

Journal

Archives of Biochemistry and Biophysics

Band

465

Seiten

336-346

Anzahl der Seiten

11

ISSN

0003-9861

Publikationsdatum

15.09.2007

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biophysik, Biochemie, Molekularbiologie

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1016/j.abb.2007.06.034 (Zugang: Geschlossen)